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Epidemiologia e Serviços de Saúde
versão impressa ISSN 1679-4974versão On-line ISSN 2237-9622
Resumo
PASSOS, Luiz Augusto Corrêa et al. Survival and infectivity of Trypanosoma cruzi in açaí pulp: in vitro and in vivo study. Epidemiol. Serv. Saúde [online]. 2012, vol.21, n.2, pp.223-232. ISSN 1679-4974. http://dx.doi.org/10.5123/S1679-49742012000200005.
OBJECTIVE: to evaluate infectivity by Trypanosoma cruzi and its survival in açaí pulp. METHODS: experiments were performed in vitro and in vivo; aliquots of pulp from Belém City (capital of the state of Pará, Northern Region of Brazil) were mixed with 105 trypomastigotes, and maintained at room temperature, and at temperatures of 4oC and -20oC, within different incubation periods; following that, the parasites were isolated and inoculated into immunodeficient mice CB-17-Prkdcscid/PasUnib, by intraperitoneal, oral, orgavage routes. RESULTS: all routes showed effectiveness. There was a delay of 5 days in the beginning of the parasitemia by oral infection; the pulp at room temperature for 48h kept the parasite virulence preserved. Keeping the samples cooled at 4oC during 144h and the freezing at -20oC for 26h did not kill the parasite. CONCLUSION: tests suggest that açaí pulp consumption may be related to outbreaks of acute Chagas' disease in Northern Brazil.
Palavras-chave : Trypanosoma cruzi; Chagas Disease; Juices; Mice; Imunocompromised Host.