Abstract

FRANCO, Regina Maura Bueno et al. Performance evaluation of different methodologies for detection of Cryptosporidium spp. and Giardia spp. in water for human consumption to meet the demands of the Environmental Health Surveillance in Brazil. Epidemiol. Serv. Saúde [online]. 2012, vol.21, n.2, pp.233-242. ISSN 1679-4974.  http://dx.doi.org/10.5123/S1679-49742012000200006.

OBJECTIVE: this study aimed to evaluate three different concentration protocols of cysts and oocysts in raw water samples in Brazilian rivers. METHODS: the protocols studied were chemical precipitation, filtration in membranes of 47mm of diameter versus nominal porosity of 3µm and filtration using Filta-Max^® system. The raw water samples collectedfrom rivers in the states of São Paulo andMinas Gerais were analyzed after being seeded with Color-Seed^®. The results of initial precision using filtration in membranes and recovery of Color-Seed^® organisms were compared to the acceptance criteria established by the United States Environmental Protection Agency (USEPA). RESULTS: The study of initial precision to Giardia using filtration in membranes and recovery of Color-Seed^® met the USEPA criteria; for Cryptosporidium only the Filta-Max^® achieved the established criteria. CONCLUSION: Filta-Max^® system showed to be the only method that achieved all the performance criteria for identification of Giardia and Cryptosporidium in water.

Keywords : Cryptosporidium; Giardia; Detection Methods; Waters.

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