Abstract

MELO, Giane Zupellari dos Santos; COSTA, Cristóvão Alves da  and  SANTOS, Ilia Gilmara Carvalho dos. Group A rotavirus molecular diversity in the city of Manaus, Amazonas state, Brazil 2004-2006. Epidemiol. Serv. Saúde [online]. 2013, vol.22, n.2, pp.265-272. ISSN 1679-4974.  http://dx.doi.org/10.5123/S1679-49742013000200008.

OBJECTIVE: verify rotavirus A genotype occurrence in children aged 1 to 36 months in Manaus-AM. METHODS: descriptive and retrospective study conducted from June/2004 to December/2006, through laboratory analysis of diarrheal stools of children aged 1 to 36 months cared for at a Child Emergency service in Manaus/Amazonas. RESULTS: 25% rotavirus A positivity was found using Polyacrylamide Gel Electrophoresis of Ribonucleic Acid. Reverse Transcriptase followed by Polymerase Chain Reaction were applied to positive samples and revealed 30.7% G genotypes and 44.7% P genotypes, with predominance of G2 (17%) and P[8] (66.7%) genotypes. 38.3% and 11.7% of the G and P genotype positive samples, respectively, showed no features of any of the G and P genotypes surveyed. CONCLUSION: 38.3% and 11.7% G and P genotypes, respectively, showed no characteristics of the genotypes studied, suggesting that genotypes different to these are in circulation.

Keywords : Gastroenteritis; Rotavirus Infections; Genotyping Techniques; Epidemiology Descriptive.

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