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Revista Pan-Amazônica de Saúde

versão On-line ISSN 2176-6223

Resumo

PADULA, Paula Julieta et al. Partial genetic characterization of Seoul hantavirus in rats from Buenos Aires City, Argentina, and generation of a Seoul recombinant nucleoprotein antigen. Rev Pan-Amaz Saude [online]. 2010, vol.1, n.2, pp.97-103. ISSN 2176-6223.  http://dx.doi.org/10.5123/S2176-62232010000200012.

Hemorrhagic fever with renal syndrome (HFRS) is a severe infectious disease characterized by fever, hemorrhage, renal impairment, and thrombocytopenia. At least seven hantaviruses cause HFRS: Hantaan, Seoul (SEOV) (distributed worldwide), Dobrava/Belgrade, Saaremaa, Amur, Thailand and Puumala. To investigate the epidemiology of HFRS and virus transmission in Argentina, we constructed a prokaryotic plasmid encoding the SEOV rNP, of 430 amino acids. After expression, the rNP was tested as an antigen for use in an enzyme-linked immunosorbent assay for infection diagnosis. To determine the current level of virus transmission in wild brown rats or Norway rats (Rattus norvegicus) captured in Buenos Aires City, Argentina, we tested tissues from rats that were determined to be serologically positive for the SEOV, and the viral genome were detected by RT-PCR using specific primers for two fragments of M segment-encoding Gn and Gc proteins. The viral genome was detected in 11 of 21 seropositive rats (52.4%) captured in two parklands. Sequence analysis of a 333-nt region of the Gc-encoding M segment revealed 97% and 96% identity with strains of SEOV from Baltimore and Brazil, respectively. Our genetic data confirm a very low diversity among SEOV virus strains.

Palavras-chave : Hantavirus; Seoul virus; Rats; Prokaryotic Cells; Recombinant Proteins; Enzyme-Linked Immunosorbent Assay.

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