Abstract

GAMARRA, Maria Liz et al. Susceptibility of a continuous murine cell line (GRX) to viral infection. Rev Pan-Amaz Saude [online]. 2011, vol.2, n.2, pp.65-69. ISSN 2176-6215.  http://dx.doi.org/10.5123/S2176-62232011000200009.

The ability of a murine cell line (GRX) to support viral replication was evaluated. GRX cell cultures were infected with different DNA or RNA viruses. It was observed that the GRX cell line is susceptible to the replication of Herpes simplex virus types 1 and 2 (HSV-1 and HSV-2), Mayaro virus (MAY), Sindbis virus (SIN), and West equine encephalitis virus (WEE), and can be used as substrate for viral replication studies. Viral replication induced cytopathic effect (CPE) 24-48 h post-infection. The GRX cells yielded infectious virus titers between 10^2.4 TCID₅₀ (Tissue Culture Infectious Dose₅₀) /25 µL and 10^5.4 TCID₅₀/25 µL in the first viral passage. These results demonstrate that GRX cells efficiently sustain viralreplication and therefore can be used as a valuable tool in the virology laboratory.

Keywords : Cell culture technique; Hepatic Stellate Cells (GRX); Virus replication.

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