SciELO - Scientific Electronic Library Online

 
vol.2 número2Desenvolvimento de uma vacina tetravalente contra dengueCaracterização molecular do parvovirus humano B19 associado com neuromielite índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

  • Não possue artigos citadosCitado por SciELO

Links relacionados

  • Não possue artigos similaresSimilares em SciELO

Compartilhar


Revista Pan-Amazônica de Saúde

versão On-line ISSN 2176-6223

Resumo

GAMARRA, Maria Liz et al. Susceptibility of a continuous murine cell line (GRX) to viral infection. Rev Pan-Amaz Saude [online]. 2011, vol.2, n.2, pp.65-69. ISSN 2176-6223.  http://dx.doi.org/10.5123/S2176-62232011000200009.

The ability of a murine cell line (GRX) to support viral replication was evaluated. GRX cell cultures were infected with different DNA or RNA viruses. It was observed that the GRX cell line is susceptible to the replication of Herpes simplex virus types 1 and 2 (HSV-1 and HSV-2), Mayaro virus (MAY), Sindbis virus (SIN), and West equine encephalitis virus (WEE), and can be used as substrate for viral replication studies. Viral replication induced cytopathic effect (CPE) 24-48 h post-infection. The GRX cells yielded infectious virus titers between 102.4 TCID50 (Tissue Culture Infectious Dose50) /25 µL and 105.4 TCID50/25 µL in the first viral passage. These results demonstrate that GRX cells efficiently sustain viralreplication and therefore can be used as a valuable tool in the virology laboratory.

Palavras-chave : Cell culture technique; Hepatic Stellate Cells (GRX); Virus replication.

        · resumo em Português | Espanhol     · texto em Português | Inglês | Espanhol     · Português ( pdf ) | Inglês ( pdf ) | Espanhol ( pdf )