SciELO - Scientific Electronic Library Online

vol.5 issue3Ethnobotany of the main medicinal plants commercialized in fairs and markets of São Luis, Maranhão State, BrazilGeneral features and epidemiology of emerging arboviruses in Brazil author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




  • Have no cited articlesCited by SciELO

Related links

  • Have no similar articlesSimilars in SciELO


Revista Pan-Amazônica de Saúde

On-line version ISSN 2176-6223


FERREIRA, Jessylene de Almeida et al. Detection and characterization of human adenoviruses from cases of acute flaccid paralysis in Northern Brazil. Rev Pan-Amaz Saude [online]. 2014, vol.5, n.3, pp.47-54. ISSN 2176-6223.

The human adenovirus (HAdV) cause a variety of infections such as acute respiratory, gastrointestinal, ocular, urinary tract and also serious neurological syndromes. The present study aimed to identify and characterize HAdV in stool samples of patients from the Northern region of Brazil in the period of 1998 to 2012, with acute flaccid paralysis, negative for poliovirus and enterovirus nonpolio. In this study, 19 samples showed a characteristic cytopathic effect of HAdV when inoculated in HEp2-C and L20B lineages. These samples were subjected by the polymerase chain reaction (PCR) in real-time (qPCR) and partial nucleotide sequencing of the hexon gene. Of the total samples, 13 were confirmed by qPCR, PCR and nucleotide sequencing. Genetic analysis showed the following viral characterization: one sample of HAdV-F41; three samples of HAdV-A31 and nine species C, three of HAdV-C5-C6 and six of HAdV The positive samples were from the States of Pará (6/19 - 31.6%), Amazonas (6/19 -31.6%) and Acre (1/19 - 5.2%). In relation to the age group, from 13 positive cases, 12 were children under 5 years old, representing 92.3% of cases. The results suggest a possible HAdV participation in the etiology of acute flaccid paralysis occurred in Northern Brazil.

Keywords : Adenoviruses, Human; Acute Flaccid Paralysis; Real-Time Polymerase Chain Reaction; Cell Culture Techniques.

        · abstract in Portuguese | Spanish     · text in Portuguese     · Portuguese ( pdf )