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Revista Pan-Amazônica de Saúde

versão impressa ISSN 2176-6223versão On-line ISSN 2176-6223

Resumo

CARNEIRO, Liliane Almeida et al. Serological, molecular and pathological investigation for leptospirosis in ovines (Ovis aries) coming from a vivarium creation. Rev Pan-Amaz Saude [online]. 2015, vol.6, n.4, pp.55-61. ISSN 2176-6223.

The current paper reports an outbreak of leptospirosis in sheep production created with the purpose of blood supply to laboratories for production of growth medium and complement fixation test. Serological, molecular and histopathological analyses were performed. Serologic analysis was performed on 12 samples using the microscopic agglutination test with cutoff point of 100 for the serovars: Leptospira Bataviae, L. Javanica, L. Panama, L. Hebdomadis, L. Castellonis, L. Icterohaemorrhagiae, L. Pyrogenes, L. Cynopteri, L. Serjoe, L. Australis, L. Wolffi, L. Copenhageni, L. Autumnalis, L. Pomona e L. Tarassovi. Anatomopathological examination was performed on only one animal, which was found dead and molecular analyzes were performed in its blood and urine using the qPCR technique. 50% of those animals were reagents to the serological diagnosis, being identified the serovars: L. Copenhageni (1/100 e 1/200), L. Castellonis (1/200 e 1/200), L. Icterohaemorrhagiae (1/200) e L. Cynopteri (1/200). The necropsied animal presented bleeding, jaundice and hemoglobinuria. Molecular analysis showed three positive animals in blood samples and two positive animals in urine samples, with no concomitant positivity for each animal. The identification of Icterohaemorrhagiae and Copenhageni serovars suggests the need for studies aiming the agent isolation in the Brazilian Amazon Region and characterization of its pathogenicity since in Brazil these are the main etiologic agents of leptospirosis cases in humans.

Palavras-chave : Leptospira; Sheep; Vivarium.

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