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Revista Pan-Amazônica de Saúde
versión impresa ISSN 2176-6215versión On-line ISSN 2176-6223
Resumen
LIMA, Dayane Pereira de; MENDES, Gabriella da Silva y SANTOS, Norma. Isolation, typing, and acyclovir susceptibility of human herpesvirus 1 and 2 detected in Rio de Janeiro, Brazil. Rev Pan-Amaz Saude [online]. 2023, vol.14, e202301253. Epub 15-Mar-2023. ISSN 2176-6215. http://dx.doi.org/10.5123/s2176-6223202301253.
OBJECTIVES:
To isolate and identify human herpesvirus (HHV) strains circulating in healthy individuals in Rio de Janeiro, Brazil, and characterize their acyclovir (ACV) susceptibility.
MATERIALS AND METHODS:
Fifteen swabs from cutaneous or mucosal lesions were used to inoculate Vero cells for viral propagation. The species of the isolates were identified by amplifying and sequencing a portion of the conserved region of the herpesvirus DNA polymerase gene. ACV susceptibility was determined using dose-response assays.
RESULTS:
HHV strains were isolated from all 15 samples. Ten isolates belonged to species HHV-1 and five to species HHV-2. Susceptibility assays showed that 13 isolates were sensitive to ACV, one had intermediate resistance, and one was resistant.
CONCLUSION:
The results presented in this study demonstrate the circulation of HHV-1 and HHV-2 in healthy individuals in Rio de Janeiro, including ACV-resistant strains, and highlight the need for continued surveillance in the face of the emergence of HHV strains resistant to antiviral drugs used in the treatment of these infections.
Palabras clave : Human Herpesvirus 1; Human Herpesvirus 2; Acyclovir; Drug Resistance, Viral.