Services on Demand
Journal
Article
Portuguese (pdf)
Article in xml format
Article references
Automatic translation
Send this article by e-mailIndicators
Cited by SciELO
Related links
Similars in
SciELO
Share
Permalink
Revista Pan-Amazônica de Saúde
Print version ISSN 2176-6215On-line version ISSN 2176-6223
Abstract
OLIVEIRA, Andreza Paloma Góes; GRACAS, Diego Assis das; BARAUNA, Rafael Azevedo and SILVA, Artur Luiz da Costa da. High abundance and diversity of antibiotic resistance genes during the Amazonian winter in the reservoir of the Tucuruí Hydroelectric Plant, state of Pará, Brazil. Rev Pan-Amaz Saude [online]. 2025, vol.16, e202501779. Epub Nov 18, 2025. ISSN 2176-6215. http://dx.doi.org/10.5123/s2176-6223202501779.
Tucuruí is the second largest fully Brazilian hydroelectric plant, and the artificial lake formed after its construction profoundly altered the regional ecosystem. Many pathogenic and free-living microorganisms that harbor antibiotic resistance genes (ARGs) are adapted to aquatic habitats, making rivers potential reservoirs of ARGs.
OBJECTIVE:
To describe the occurrence of ARGs according to spatial and seasonal variability in the Tucuruí Hydroelectric Plant reservoir.
MATERIALS AND METHODS:
Samples were collected from the photic, aphotic, and sediment layers at the Montante 1 and Montante Novo Repartimento stations during the Amazonian summer and winter. DNA was extracted and sequenced on the Ion Proton platform for metagenomic analysis. De novo assembly was performed using MEGAHIT, open reading frames were predicted with Prodigal, and ARGs were identified using the CARD and MEGARes databases. Contigs containing ARGs were generated for subsequent analyses. Statistical analyses were conducted in R using the ggplot2 package.
RESULTS:
The most abundant ARGs in the samples were those related to beta-lactams (3,317.92 ×/Gb), macrolide-lincosamide-streptogramin (1,079.77 ×/Gb), tetracyclines (968.23 ×/Gb), aminoglycosides (779.25 ×/Gb), and multidrug resistance genes (698.94 ×/Gb). These ARGs occurred predominantly during the Amazonian winter in the surface layer of the reservoir.
CONCLUSION:
The outcomes screen significant anthropogenic influence and concern regarding the spread of antibiotic resistance genes in the Tucuruí Hydroelectric Plant reservoir.
Keywords : Genes, MDR; Metagenomics; Hydroelectric Power Plants (Environmental Health); Amazonian Ecosystem.
