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Revista Pan-Amazônica de Saúde

versão impressa ISSN 2176-6215versão On-line ISSN 2176-6223

Rev Pan-Amaz Saude v.2 n.1 Ananindeua mar. 2011

http://dx.doi.org/10.5123/S2176-62232011000100014 

SUMMARY OF THESIS AND DISSERTATION | RESUMO DE TESE E DISSERTAÇÃO | RESUMEN DE TESIS Y DISERTACIÓN

 

Molecular characterization of G and P rotavirus genotypes, G9 strain, from children with acute gastroenteritis in the Metropolitan Region of Belém, Pará State, from 1999 to 2007

 

Caracterização molecular dos genótipos G e P de rotavírus, cepa G9, de crianças com gastrenterite aguda na Região Metropolitana de Belém, Estado do Pará, de 1999 a 2007

 

Caracterización molecular de los genotipos G y P de rotavirus cepa G9, de niños con gastroenteritis aguda en la Región Metropolitana de Belém, Estado de Pará, de 1999 a 2007

 

 

Sylvia de Fátima Santos Guerra; Luana da Silva Soares; Joana D'Arc Pereira Mascarenhas

Instituto Evandro Chagas/SVS/MS, Ananindeua, Pará, Brasil

Endereço para correspondência
Correspondence
Dirección para correspondencia

 

 


ABSTRACT

Rotavirus (RV) is the major viral agent associated with gastroenteritis and is responsible for 39% of all diarrheal cases requiring hospitalization, as well as approximately 520 thousand deaths among children under five years of age. Rotavirus belongs to the Reoviridae family, genus Rotavirus and its genome consists of 11 segments of double-stranded RNA which encode six structural viral proteins (VPs) and six non-structural viral proteins (NSPs). VP4 and VP7 proteins make up the outer layer of the RV particle and define the two genotypes, P and G, respectively. To date, at least 23 G genotypes and 31 P genotypes have been identified. G9-type RV strains, which are associated with a more severe disease in humans, have emerged globally. Dual type-specificities have been reported, and G9 strains most often belong to the P[8] genotype. While G9 genotype includes six distinct lineages, four distinct lineages are currently recognized as belonging to the P[8] genotype. The aim of this study was to characterize the VP4 and VP7 genes of the RV G9 genotypes that circulated in the Metropolitan Region of Belém, Pará, in northern Brazil from 1999 to 2007. The viral dsRNA of 38 samples was extracted from fecal suspensions and analyzed using polyacrylamide gel electrophoresis to determine electropherotypes, followed by sequencing reactions. Overall, 32 selected G9P[8] samples were analyzed, all of which possessed long electropherotype. Phylogenetic analysis of the VP7 gene showed that all G9 strains belonged to lineage 3 with a high degree of similarity between them; only eight nucleotide changes have been detected in this lineage. However, only three of these amino acid substitutions were observed in our study, occurring at positions 43 (lV), 66 (AV) and 73 (QR). Of interest, the substitutions at positions 43 and 73 were only found in samples from 2007. Phylogenetic analysis of the VP4 gene revealed that all of the P[8] strains belonged to lineage 3, with 15 nucleotide changes corresponding to four amino acid substitutions at positions 108 (Vl), 172 (RK), 173 (lV) and 275 (KR). The amino acid substitutions at positions 172 and 275 were observed only in RV isolates from 1999 to 2002. Overall, the RV G9 samples showed high homology throughout the entire study period. Interestingly, strains isolated in 2007 were the most divergent with respect to both VP4 and VP7 genes. Our data underscore the need for continuous surveillance of circulating RV strains in our region to detect the emergence of new genetic variants that would pose a challenge to current RV immunization strategies. This should include close monitoring of the genetic diversity of emerging G9 strains.

Keywords: Genetic Variation; Genotype; Gastroenteritis.


 

 

Correspondencia /Correspondência /Correspondence
Sylvia de Fátima Santos Guerra
Instituto Evandro Chagas
Rodovia BR 316, km 7, s/no, Levilândia
CEP:67030-000
Ananindeua-Pará-Brasil
Tel: +55 (91) 3214-2025
E-mail:syviaguerra@iec.pa.gov.br

Recebido em / Received / Recibido en: 24/11/2010
Aceito em / Accepted / Aceito en: 25/2/2011