<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2176-6223</journal-id>
<journal-title><![CDATA[Revista Pan-Amazônica de Saúde]]></journal-title>
<abbrev-journal-title><![CDATA[Rev Pan-Amaz Saude]]></abbrev-journal-title>
<issn>2176-6223</issn>
<publisher>
<publisher-name><![CDATA[Instituto Evandro Chagas. Secretaria de Vigilância em Saúde e Ambiente. Ministério da Saúde]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2176-62232025000110115</article-id>
<article-id pub-id-type="doi">10.5123/s2176-6223202501785</article-id>
<title-group>
<article-title xml:lang="pt"><![CDATA[Composição microbiana da Praia do Amor, Ilha de Caratateua, Distrito de Outeiro, estado do Pará, Brasil]]></article-title>
<article-title xml:lang="en"><![CDATA[Microbial composition of Praia do Amor Beach, Caratateua Island, Outeiro District, Pará State, Brasil]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Monteiro]]></surname>
<given-names><![CDATA[Carolina Costa]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Santos]]></surname>
<given-names><![CDATA[Estérfane Monique de Oliveira]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Deus]]></surname>
<given-names><![CDATA[Danielle Rodrigues de]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Teixeira]]></surname>
<given-names><![CDATA[Dielle Monteiro]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Carneiro]]></surname>
<given-names><![CDATA[Bruno Santana]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Soares]]></surname>
<given-names><![CDATA[Luana Silva]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Silva]]></surname>
<given-names><![CDATA[Mônica Cristina de Moraes]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Siqueira]]></surname>
<given-names><![CDATA[Jones Anderson Monteiro]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Centro Universitário Metropolitano da Amazônia Curso de Bacharelado em Biomedicina ]]></institution>
<addr-line><![CDATA[Belém Pará]]></addr-line>
<country>Brasil</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Instituto Evandro Chagas Seção de Virologia Laboratório de Virologia Ambiental]]></institution>
<addr-line><![CDATA[Ananindeua Pará]]></addr-line>
<country>Brasil</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Instituto Evandro Chagas Seção de Virologia Laboratório de Bioinformática]]></institution>
<addr-line><![CDATA[Ananindeua Pará]]></addr-line>
<country>Brasil</country>
</aff>
<aff id="Af4">
<institution><![CDATA[,Instituto Evandro Chagas Seção de Virologia Laboratório de Vírus Gastroentéricos]]></institution>
<addr-line><![CDATA[Ananindeua Pará]]></addr-line>
<country>Brasil</country>
</aff>
<aff id="Af5">
<institution><![CDATA[,Instituto Evandro Chagas Seção de Meio Ambiente Laboratório de Microbiologia Ambiental]]></institution>
<addr-line><![CDATA[Ananindeua Pará]]></addr-line>
<country>Brasil</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>00</month>
<year>2025</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>00</month>
<year>2025</year>
</pub-date>
<volume>16</volume>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.iec.gov.br/scielo.php?script=sci_arttext&amp;pid=S2176-62232025000110115&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.iec.gov.br/scielo.php?script=sci_abstract&amp;pid=S2176-62232025000110115&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.iec.gov.br/scielo.php?script=sci_pdf&amp;pid=S2176-62232025000110115&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="pt"><p><![CDATA[RESUMO A disseminação viral em ambientes aquáticos é ampla e frequentemente supera a abundância bacteriana. No Brasil, a avaliação da qualidade das águas doces destinadas à recreação baseia-se em indicadores bacterianos, como os coliformes termotolerantes, que apresentam baixa especificidade para contaminação humana e risco viral. A Ilha de Caratateua, na Região Metropolitana de Belém, Pará, é um balneário popular, porém marcado pela ausência de saneamento básico e pelo despejo direto de esgoto nas praias, o que eleva os riscos à saúde pública.  OBJETIVO:  Avaliar a diversidade viral e microbiana da Praia do Amor, localizada na Ilha de Caratateua, Distrito de Outeiro, Pará, durante um período de monitoramento mensal, entre outubro de 2023 e setembro de 2024.  MATERIAIS E MÉTODOS:  Foram realizadas coletas mensais entre outubro de 2023 e setembro de 2024. A detecção viral foi conduzida por reação em cadeia da polimerase (PCR), e a caracterização metagenômica, por sequenciamento de nova geração.  RESULTADOS:  Detectou-se CrAssfago em 91,6% das amostras, rotavírus em 41,6%, e astrovírus e adenovírus em 8,3%. A análise do microbioma revelou 13 famílias virais, com destaque para Kyanoviridae, Straboviridae e Autographviridae, e 521 famílias bacterianas, sendo Vibrionaceae, Microbacteriaceae e Pseudomonadaceae as mais prevalentes.  CONCLUSÃO:  Os resultados indicam expressiva contaminação fecal de origem humana nas águas da Praia do Amor, evidenciando a necessidade urgente de melhorias no saneamento básico dos arredores para proteger a saúde pública e o ecossistema local.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT Viral dissemination in aquatic environments is extensive and often exceeds bacterial abundance. In Brazil, the assessment of the quality of freshwater used for recreation is based on bacterial indicators, such as thermotolerant coliforms, which show low specificity for human contamination and viral risk. Caratateua Island, located in the Metropolitan Region of Belém, Pará, is a popular bathing area but is marked by the absence of basic sanitation and the direct discharge of sewage into beaches, increasing public health risks.  OBJECTIVE:  To evaluate the viral and microbial diversity of Praia do Amor Beach, located on Caratateua Island, Outeiro District, Pará, during a monthly monitoring period between October 2023 and September 2024.  MATERIALS AND METHODS:  Monthly sampling was conducted between October 2023 and September 2024. Viral detection was performed by polymerase chain reaction (PCR), and metagenomic characterization was carried out by next-generation sequencing.  RESULTS: CrAssphage was detected in 91.6% of samples, rotavirus in 41.6%, and astrovirus and adenovirus in 8.3%. Microbiome analysis revealed 13 viral families, mainly Kyanoviridae, Straboviridae, and Autographviridae, and 521 bacterial families, with Vibrionaceae, Microbacteriaceae, and Pseudomonadaceae being the most prevalent.  CONCLUSION:  The results indicate significant fecal contamination of human origin in the waters of Praia do Amor Beach, highlighting the urgent need for improved sanitation in the surrounding area to protect public health and the local ecosystem.]]></p></abstract>
<kwd-group>
<kwd lng="pt"><![CDATA[Metagenômica]]></kwd>
<kwd lng="pt"><![CDATA[Viroma]]></kwd>
<kwd lng="pt"><![CDATA[Doenças Transmitidas pela Água]]></kwd>
<kwd lng="pt"><![CDATA[Ecossistema Amazônico]]></kwd>
<kwd lng="en"><![CDATA[Metagenomics]]></kwd>
<kwd lng="en"><![CDATA[Virome]]></kwd>
<kwd lng="en"><![CDATA[Waterborne Diseases]]></kwd>
<kwd lng="en"><![CDATA[Amazonian Ecosystem]]></kwd>
</kwd-group>
</article-meta>
</front><back>
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