<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2176-6223</journal-id>
<journal-title><![CDATA[Revista Pan-Amazônica de Saúde]]></journal-title>
<abbrev-journal-title><![CDATA[Rev Pan-Amaz Saude]]></abbrev-journal-title>
<issn>2176-6223</issn>
<publisher>
<publisher-name><![CDATA[Instituto Evandro Chagas. Secretaria de Vigilância em Saúde e Ambiente. Ministério da Saúde]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2176-62232025000110123</article-id>
<article-id pub-id-type="doi">10.5123/s2176-6223202501786</article-id>
<title-group>
<article-title xml:lang="pt"><![CDATA[Diagnóstico ambiental de SARS-CoV-2: correlação viral com fatores bióticos e abióticos em águas superficiais do centro urbano de Belém, estado do Pará, Brasil]]></article-title>
<article-title xml:lang="en"><![CDATA[Environmental diagnosis of SARS-CoV-2: viral correlation with biotic and abiotic factors in surface waters of the urban center of Belém, Pará State, Brazil]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Batista]]></surname>
<given-names><![CDATA[Gabrielly Ketenen Costa]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Santos]]></surname>
<given-names><![CDATA[Denise Suéllen Amorim de Sousa]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Coimbra]]></surname>
<given-names><![CDATA[Vanessa Brenda Silva]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Luz]]></surname>
<given-names><![CDATA[Breno Moreira da]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Sagica]]></surname>
<given-names><![CDATA[Fernanda do Espírito Santo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Siqueira]]></surname>
<given-names><![CDATA[Jones Anderson Monteiro]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Morais]]></surname>
<given-names><![CDATA[Lena Líllian Canto de Sá]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Programa de Pós-Graduação em Epidemiologia e Vigilância em Saúde  ]]></institution>
<addr-line><![CDATA[Ananindeua Pará]]></addr-line>
<country>Brasil</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Instituto Evandro Chagas Seção de Meio Ambiente ]]></institution>
<addr-line><![CDATA[Ananindeua Pará]]></addr-line>
<country>Brasil</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Instituto Evandro Chagas Seção de Virologia ]]></institution>
<addr-line><![CDATA[Ananindeua Pará]]></addr-line>
<country>Brasil</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>00</month>
<year>2025</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>00</month>
<year>2025</year>
</pub-date>
<volume>16</volume>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.iec.gov.br/scielo.php?script=sci_arttext&amp;pid=S2176-62232025000110123&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.iec.gov.br/scielo.php?script=sci_abstract&amp;pid=S2176-62232025000110123&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.iec.gov.br/scielo.php?script=sci_pdf&amp;pid=S2176-62232025000110123&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="pt"><p><![CDATA[RESUMO  OBJETIVO:  monitorar a ocorrência do RNA do SARS-CoV-2 em águas superficiais de canais urbanos da cidade de Belém, estado do Pará, entre 2021 e 2022, correlacionando os achados com parâmetros ambientais, indicadores microbiológicos e dados epidemiológicos.  MATERIAIS E MÉTODOS:  foram realizadas 24 coletas mensais em oito pontos, totalizando 192 amostras. As amostras foram analisadas quanto a parâmetros físico-químicos por meio de sonda multiparamétrica, presença de coliformes termotolerantes (CTT) e Escherichia coli pelo método ONPG-MUG, além da detecção viral por RT-qPCR direcionada aos genes N e ORF1ab.  RESULTADOS:  Todas as amostras apresentaram concentrações de CTT e E. coli acima dos limites estabelecidos pela Resolução CONAMA nº 274/2000. O RNA do SARS-CoV-2 foi detectado em 23,44% das amostras para o gene N e em 11,45% para o gene ORF1ab, com maiores taxas nos primeiros meses do ano, coincidindo com os picos de casos de COVID-19. O gene ORF1ab apresentou correlação significativa com casos e óbitos (rs = 0,73595; p = 0,0064 e rs = 0,78101; p = 0,0027 em 2021) e explicou 81,69% da variação no número de casos (R2 = 0,8169). A temperatura foi a única variável ambiental com correlação significativa com o gene N, enquanto não se observaram associações com os indicadores microbiológicos.  CONCLUSÃO:  A detecção do SARS-CoV-2 em águas superficiais de Belém evidencia riscos à saúde pública, especialmente em áreas com saneamento precário, reforçando a virologia ambiental como ferramenta estratégica para vigilância epidemiológica e para subsidiar políticas de prevenção e mitigação de riscos.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT  OBJECTIVE:  To monitor the occurrence of SARS-CoV-2 RNA in surface waters of urban canals in the city of Belém, state of Pará, between 2021 and 2022, correlating the findings with environmental parameters, microbiological indicators, and epidemiological data.  MATERIALS AND METHODS:  Twenty-four monthly sampling campaigns were conducted at eight sites, totaling 192 samples. The samples were analyzed for physicochemical parameters using a multiparameter probe, the presence of thermotolerant coliforms (TTC) and Escherichia coli by the ONPG-MUG method, and viral detection by RT-qPCR targeting the N and ORF1ab genes.  RESULTS:  All samples showed TTC and E. coli concentrations above the limits established by CONAMA Resolution No. 274/2000. SARS-CoV-2 RNA was detected in 23.44% of samples for the N gene and 11.45% for the ORF1ab gene, with higher detection rates in the early months of the year, coinciding with peaks in COVID-19 cases. The ORF1ab gene showed a significant correlation with cases and deaths (rs = 0.73595; p = 0.0064 and rs = 0.78101; p = 0.0027 in 2021) and accounted for 81.69% of the variation in case numbers (R2 = 0.8169). Temperature was the only environmental variable significantly correlated with the N gene, while no associations were observed with microbiological indicators.  CONCLUSION:  The detection of SARS-CoV-2 in surface waters of Belém highlights public health risks, particularly in areas with inadequate sanitation, reinforcing the role of environmental virology as a strategic tool for epidemiological surveillance and for informing policies aimed at risk prevention and mitigation.]]></p></abstract>
<kwd-group>
<kwd lng="pt"><![CDATA[SARS-CoV-2]]></kwd>
<kwd lng="pt"><![CDATA[Infecções Respiratórias]]></kwd>
<kwd lng="pt"><![CDATA[Monitoramento Ambiental, Monitoramento Biológico, Enterobacteriaceae]]></kwd>
<kwd lng="en"><![CDATA[SARS-CoV-2]]></kwd>
<kwd lng="en"><![CDATA[Respiratory Tract Infections]]></kwd>
<kwd lng="en"><![CDATA[Environmental Monitoring, Biological Monitoring, Enterobacteriaceae]]></kwd>
</kwd-group>
</article-meta>
</front><back>
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